Chikungunya Virus Capsid Protein

Size

£1,675.00

SKU:: 506229
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Native Chikungunya virus.
Clone:: CA980
Format:: Supplied as a liquid in in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Chikungunya virus is the aetiological agent of chikungunya fever. CHIKV belongs to the Alphavirus genus, and is an enveloped, single-stranded positive-sense RNA virus (Strauss & Strauss, 1994). The alphavirus genome encodes four nonstructural proteins (nsP1 to nsP4) and five structural proteins (capsid, E3, E2, 6K and E1). CHIKV is transmitted to humans by Aedes mosquitoes, and disease is characterized by a rapid onset of fever, myalgia and often a rash (usually maculopapular), with chronic disease characterized by episodic, and often debilitating, polyarthralgia/polyarthritis. (Suhrbier et al., 2012). The largest epidemic of CHIKV disease ever reported began in 2004 and has since been responsible for up to 6.5 million human cases, primarily in Africa and Asia, with imported cases reported in over 40 countries. CHIKV infection is symptomatically similar to infection with Zika virus and Dengue virus, and differential diagnosis using immunoassay based testing is important in patient management. The alphavirus capsid protein (CP) is a multifunctional protein that has been shown to act as a serine protease for self-cleavage, binds viral genomic RNA and other CP molecules during nucleocapsid formation, and interact with viral spike proteins during virion formation and egress (Choi et al., 1991). The CP of CHIKV forms two major domains. The N-terminal domain is implicated in non-specific RNA binding, while the C-terminal domain harbors the globular protease and the binding site for the spike protein (Hong et al., 2006). Applications: Suitable for use in ELISA and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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