Elongin B, Recombinant, Mouse, aa1-118, His-Tag (Elob)

Size

£544.00

SKU:: 506168
Molecular Weight:: 15.6141
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 10% glycerol.
Extra Details:: Elob, also known as elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimeric consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant protein corresponding to aa1-118 of mouse Elob, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~15.6kD (141aa), confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDVFLMI RRHKTTIFTD AKESSTVFEL KRIVEGILKR PPEEQRLYKD DQLLDDGKTL GECGFTSQTA RPQAPATVGL AFRADDTFEA LRIEPFSSPP ELPDVMKPQD SGGSANEQAV Q Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.