MYPT1, phosphorylated (Thr696)

Size

£908.00

SKU:: 505905
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human MYPT1 around the phosphorylation site of T696. Species sequence homology: Zebrafish, Bovine, Equine, Sheep, Rabbit, Canine, Chicken and, Xenopus
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Extra Details:: Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. MYPT1 promotor contains one Sp1 transcription factor binding site, suggesting that MYPT1 is a housekeeping gene. Myotonic dystrophy protein kinase phosphorylates MYPT1 at Tyrosine 654 to regulate Myosin II phosphorylation. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphoryl-ates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated Myosin light chain to MYPT1. PP1c interacts with at least four binding sties on the N-terminus of MYPT1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice