501508
DANRE Stk3 (36kD Subunit), ID
Size
£696.00
- SKU:
- 501508
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated synthetic peptide mapping to a fragment of residues within aa228-252 of the Central region of human DANRE stk3.
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein stk3/mst2 and stk4/mst1, in complex with its regulatory protein sav1, phosphorylates and activates lats1/2 in complex with its regulatory protein mob1, which in turn phosphorylates and inactivates yap1 oncoprotein and wwtr1/taz. Phosphorylation of yap1 by lats2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (By similarity). Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice