ATG4A

Size

£696.00

SKU:: 501211
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human ATG4A.
Clone:: 1458CT808.66.25.69
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminal is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. Applications: Suitable for use in Immunofluorescence, Indirect Flow Cytometry, ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:25 Indirect Flow Cytometry: 1:25 Western Blot: 1:500 Immunohistochemistry (Paraffin): 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.