Varicella Zoster Virus, Nucleocapsid (VZV)

Size

£927.00

SKU:: 497285
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: VZV Ellen Strain from BSC-1 cells
Clone:: 22G1
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Varicella zoster virus (VZV) is one of eight herpes viruses known to infect humans and other vertebrates. It commonly causes chicken-pox in children and adults and herpes zoster (shingles) in adults and rarely in children. As with the other herpesviruses, VZV causes both acute illness and lifelong latency. Before vaccination became widespread, acute primary infection (varicella or "chickenpox") was common during childhood--especially in temperate climates. Primary infection is much less common in recent years as a result of childhood vaccination, but still may occur in unvaccinated individuals and in instances of vaccine failure. Varicella usually is a benign and self-limiting illness, but can be more severe in adults and in individuals with cellular immunodeficiency. These individuals are at much higher risk of pneumonia and disseminated disease with visceral involvement. Zoster typically presents as a painful, localized cutaneous eruption occurring along 1 or more contiguous dermatomes. As with varicella, zoster usually is self-limited in the immunocompetent host, but immunocompromised persons are at risk of more severe illness with cutaneous or visceral dissemination. Pain is a frequent complication of zoster, and pain that persists following complete healing of cutaneous lesions, referred to as post-therapeutic neuralgia, can be debilitating and difficult to control. VZV genome is a linear duplex DNA molecule, a laboratory strain has 124 Kb. VZV virons are spherical and 180–200nm in diameter. Their lipid envelope encloses the 100nm nucleocapsid of 162 hexameric and pentameric capsomeres arranged in an icosahedral form. VZV is closely related to the herpes simplex viruses (HSV), sharing much genome homology. The known envelope glycoproteins (gB, gC, gE, gH, gI, gK, gL) correspond with those in HSV; however, there is no equivalent of HSV gD. There are at least five clades of this virus. Clades 1 and 3 include European/North American strains; clade 2 are Asian strains, especially from Japan; and clade 5 appears to be based in India. Clade 4 includes some strains from Europe but its geographic origins need further clarification. Varivax (Merck) is a chickenpox vaccine for children, adolescents and adults. Zostavax is a more concentrated formulation of the Varivax vaccine, designed to elicit an immune response in older adults whose immunity to VZV wanes with advancing age. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1-10ug/ml Immunoprecipitation: 1-10ug/ml Immunohistochemistry: 1-10ug/ml Optimal dilutions to be determined by the researcher. Negative Control: Non-immune Mouse IgG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice