IL-21, Recombinant, Canine, aa18-146

Size

£342.00

SKU:: 497044
Molecular Weight:: 15130
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU per 1ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: IL-21, also known as Interleukin-21, is an important modulator of natural killer (NK) cell function. However, little is known about IL-21 function in canine NK cells because the phenotype of these cells remains undefined. NK cells proliferated rapidly in response to activation by IL-21 for 3 weeks, and IL-21 was able to induce changes in the mRNA expression of NK cell-related receptors and enhance the effector function of NK cells in perforin- and granzyme-B-dependent manners. The duration, frequency and timing of IL-21 stimulation during culture affected the rate of proliferation, patterns of receptor expression, cytokine production, and anti-tumor activity. It has a potential predicating that synergistic interactions of IL-21 with IL-2 and IL-15 play an important role in the proliferation, receptor expression, and effector function of canine NK cells. Recombinant protein corresponding to aa18-146 from canine IL-21, expressed in E. coli. Molecular Weight: ~15kD Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 2ng/ml. Amino Acid Sequence: MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice