IL-1 beta/IL-1F2, Recombinant, Canine, aa114-265

Size

£351.00

SKU:: 497042
Molecular Weight:: 17.5153
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa114-265 from canine IL-1 beta/IL-1F2, expressed in E. coli. Molecular Weight: ~17.5kD (153aa) confirmed by MALDI-TOF Biological Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤5pg/ml. Amino Acid Sequence: MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice