CD39L2, Recombinant, Mouse, aa33-455, His-Tag

Size

£399.00

SKU:: 497025
Molecular Weight:: 47.3433
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Extra Details:: CD39L2, also known as ENTPD6, is a nucleoside phosphohydrolase of the CD39 family of enzymes that is present on the surface of cells. It is soluble monomeric enzyme, making it more amenable to thorough structural and fuctional analysis than the membrane-bound forms. Soluble CD39L2 preferentially exhibits nucleoside diphosphatase activity, which is depedent on the presence of divalent cations. It is expressed at its highest levels in cardiac muscle and in heart capillary endothelial cells. Also, it plays a role in the regulation and recruitment of platelet activation in heart. Recombinant mouse CD39L2, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques, Recombinant protein corresponding to aa33-455 from mouse CD39L2, fused to His-Tag at C-terminal, expressed in HEK293 cells. Molecular Weight: ~47.3kD Specific Activity: >80,000pmol/min/ug Unit Definition: Defined as the amount of enzyme that hydrolyze GDP/minute at pH 7.5 at 25C. Amino Acid Sequence: <DGSM>KWHRAS AAQAFFTIAG AASGARWTQQ AFSSPGSAAR GHEVFYGIMF DAGSTGTRIH VFQFARPPGE TPTLTHETFK ALKPGLSAYA DDVEKSAQGI QELLNVAKQH IPYDFWKATP LVLKATAGLR LLPGEKAQKL LQKVKEVFKA SPFLVGDDCV SIMNGTDEGV SAWITVNFLT GSLKTPGSSS VGMLDLGGGS TQITFLPRVE GTLQASPPGH LTALQMFNRT YKLYSYSYLG LGLMSARLAI LGGVEGKPAE NDKELVSPCL SPRFRGEWEH AEVTYRISGQ KAVGLYELCA SRVSEVLRNK VHRTEEAQHV DFYAFSYYYD LAASFGLIDA EKGGSLVVGD FEIAAKYVCR TLETQPPSSP FACMDLTYIS LLLHEFGFPG DKVLKLARKI DNVETSWALG AIFHYIDSLK RQKVPAL<HHH HHH> Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice