ACE-2, Recombinant, Human, aa18-740, hIgG-His-Tag, Active

Size

£416.00

SKU:: 497016
Molecular Weight:: 110.8965
Purity:: ≥90% (SDS-PAGE). Endotoxin: ≤1EU/1ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: ACE-2, also known as Angiotensin-converting enzyme 2, is an enzyme attached to the cell membranes of cells in the lungs, arteries, heart, kidney, and intestines. This enzyme lowers blood pressure by catalysing the hydrolysis of angiotensin II into angiotensin. It counters the activity of the related angiotensin-converting enzyme (ACE) by reducing the amount of angiotensin-II and increasing Ang(1-7) making it a promising drug target for treating cardiovascular diseases. ACE-2 also serves as the entry point into cells for some coronaviruses, including HCoVNL63, SARS-CoV, and SARS-CoV-2. Recombinant protein corresponding to aa18-740 from human ACE-2, fused to hIgG-His-Tag at C-terminal, expressed in HEK293 cells. Swiss/Uniprot: Q9BYF1 Molecular Weight: ~110.8kD Biological Activity: Measured by its binding ability in a functional ELISA with SARS-CoV-2 Spike RBD. The ED50 range ≤ 100ng/ml. Specific activity is > 250 pmol/min/ug. Unit Definition: Defined as the amount of enzyme that hydrolysis 1.0 pmole of McaYVADAPK(Dnp)-OH per minute at pH 7.5, at 25°C. Amino Acid Sequence: <DGS>QSTIEEQ AKTFLDKFNH EAEDLFYQSS LASWNYNTNI TEENVQNMNN AGDKWSAFLK EQSTLAQMYP LQEIQNLTVK LQLQALQQNG SSVLSEDKSK RLNTILNTMS TIYSTGKVCN PDNPQECLLL EPGLNEIMAN SLDYNERLWA WESWRSEVGK QLRPLYEEYV VLKNEMARAN HYEDYGDYWR GDYEVNGVDG YDYSRGQLIE DVEHTFEEIK PLYEHLHAYV RAKLMNAYPS YISPIGCLPA HLLGDMWGRF WTNLYSLTVP FGQKPNIDVT DAMVDQAWDA QRIFKEAEKF FVSVGLPNMT QGFWENSMLT DPGNVQKAVC HPTAWDLGKG DFRILMCTKV TMDDFLTAHH EMGHIQYDMA YAAQPFLLRN GANEGFHEAV GEIMSLSAAT PKHLKSIGLL SPDFQEDNET EINFLLKQAL TIVGTLPFTY MLEKWRWMVF KGEIPKDQWM KKWWEMKREI VGVVEPVPHD ETYCDPASLF HVSNDYSFIR YYTRTLYQFQ FQEALCQAAK HEGPLHKCDI SNSTEAGQKL FNMLRLGKSE PWTLALENVV GAKNMNVRPL LNYFEPLFTW LKDQNKNSFV GWSTDWSPYA DQSIKVRISL KSALGDKAYE WNDNEMYLFR SSVAYAMRQY FLKVKNQMIL FGEEDVRVAN LKPRISFNFF VTAPKNVSDI IPRTEVEKAI RMSRSRINDA FRLNDNSLEF LGIQPTLGPP NQPPVS<LEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH> Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice