IL34, Recombinant, Human, aa21-242, His-Tag (IL34, C16orf77, IL-34, Interleukin-34 Isoform 1)

Size

£607.00

SKU:: 470241
Molecular Weight:: 26.3284
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-Hcl, pH 8.5, 1mM DTT, 0.15M sodium chloride, 01mM PMSF, 1mM EDTA, 30% glycerol.
Extra Details:: IL34, also known as interleukin-34 isoform 1, is a secreted cytokine. This protein is expressed in a variety of tissues richest in the spleen and stimulates the proliferation of monocytes. In a functional study, IL-34, such as CSF-1, another ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1 / 2) in human monocytes. In addition, it promoted the formation of macrophage precursor colonization units-macrophages (CFU-M) in human bone marrow cultures. Recombinant human IL34, fused to His-Tag at C-terminal, expressed in Insect Cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa21-242 from human IL34, fused to His-Tag at C-terminal, expressed in Insect Cell. Molecular Weight: ~26.3kD (231aa) ~28-40kD (SDS-PAGE under reducing conditions) Amino Acid Sequence: ADPNEPLEMW PLTQNEECTV TGFLRDKLQY RSRLQYMKHY FPINYKISVP YEGVFRIANV TRLQRAQVSE RELRYLWVLV SLSATESVQD VLLEGHPSWK YLQEVQTLLL NVQQGLTDVE VSPKVESVLS LLNAPGPNLK LVRPKALLDN CFRVMELLYC SCCKQSSVLN WQDCEVPSPQ SCSPEPSLQY AATQLYPPPP WSPSSPPHST GSVRPVRAQG EGLLPHHHHH H Endotoxin: <1EU/1ug (LAL) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice