UBE2S (Ubiquitin-conjugating Enzyme E2 S, E2 Ubiquitin-conjugating Enzyme S, E2-EPF, Ubiquitin Carrier Protein S, Ubiquitin-conjugating Enzyme E2-24kD, Ubiquitin-conjugating Enzyme E2-EPF5, Ubiquitin-protein Ligase S, UBE2S, E2EPF, OK/SW-cl.73)

Size

£394.00

SKU:: 407269
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa1-222 of human UBE2S.
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Extra Details:: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes \'Lys-11\'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating \'Lys-11\'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except \'Lys-48\'-linked polyubiquitination. Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice