UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, UBE2F, NCE2)

Size

£394.00

SKU:: 407268
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa1-185 of human UBE2F.
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Extra Details:: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.