RNGTT (mRNA-Capping Enzyme, HCAP1, HCE, Polynucleotide 5\'-Triphosphatase, mRNA 5\'-Triphosphatase, TPase, mRNA Guanylyltransferase, GTP--RNA Guanylyltransferase, GTase, CAP1A)

Size

£394.00

SKU:: 407041
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa1-260 of human RNGTT.
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Extra Details:: Bifunctional mRNA-capping enzyme exhibiting RNA 5\'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5\'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5\'-diphosphate terminal. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.