Cofilin

Size

£479.00

SKU:: 399343
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human Cofilin.
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin,thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan.Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin. Applications: Suitable for use in Western Blot, ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 ELISA: 1:40,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.