CA19-9 (CA19.9, Ovarian Tumor Antigen, Pancreatic Tumor Antigen, Sialyl Lewis a)

Size

£422.00

SKU:: 397612
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
Clone:: SPM588
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% azide
Extra Details:: CA19-9, a carbohydrate epitope expressed on a high MW (>400kD) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This MAb is superb for staining of formalin-fixed, paraffin-embedded tissues. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5-10ul/1 million cells Immunofluorescence: 0.5-1ug/ml Western Blot: 1:100-1:200 Immunohistochemistry (Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) Optimal dilution for a specific application should be determined. Positive Control: Stomach Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice