NCF1 (Neutrophil Cytosol Factor 1, NCF-1, 47kD Autosomal Chronic Granulomatous Disease Protein, 47kD Neutrophil Oxidase Factor, NCF-47K, Neutrophil NADPH Oxidase Factor 1, Nox Organizer 2, Nox-Organizing Protein 2, SH3, PX Domain-Containing Protein 1A, p47-phox, NOXO2, SH3PXD1A)

Size

£620.00

SKU:: 397487
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human NCF1, (Position: M1-D270), expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide, 4% trehalose. Reconstitute with 200ul sterile ddH2O.
Extra Details:: Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene. The protein encoded by this gene is a 47kD cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.1-0.5ug/ml Western Blot: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.