Immunoglobulin-Like Transcript 4 (ILT4, CD85d)

Size

£889.00

SKU:: 397316
Purity:: Purified by Protein A affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Glu24-His458 from human LILRB2/CD85d/ILT4, expressed in NSO cells.
Clone:: 7H14
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1-3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains that allows association with Fcγ R, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1-3). Immunoglobulin-Like Transcript 4 (ILT-4), also known as Leukocyte Immunoglobulin-like Receptor 2 (LIR-2) and Leukocyte Immunoglobulin-Like Receptor subfamily B, member 2 (LILRB2), is an Ig superfamily inhibitory receptor expressed primarily on monocytes, macrophages, and dendritic cells (DC). Binding of HLA-G to ILT4 on DC alters DC maturation, rendering them tolerogenic. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant LILRB2. Use non-reducing conditions. Flow Cytometry: 0.25ug per 10e6 cells in human peripheral blood monocytes Neutralization: Measured by its ability to neutralize LILRB2/CD85d/ILT4mediated adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line. The neutralization dose (ND50) is typically 0.2-0.8ug/ml in the presence of 35ug/ml recombinant human LILRB2/CD85d/ILT4-Fc chimera. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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