F4/80 (Gpf480) (PE) [KO Validated]

Size

£448.00

SKU:: 397072
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Clone:: Cl:A3-1
Format:: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 250ul/1ml dH2O. Labeled with R-Phycoerythrin (PE).
Extra Details:: Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed. Rat anti mouse F4/80 antibody, modulates cytokine levels released in response to Listeria monocytogenes. Applications: Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from HOB2 rats. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice