F4/80 (Epidermal Growth Factor TM7, Gpf480) (Biotin) [KO Validated]

Size

£429.00

SKU:: 397070
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Clone:: Cl:A3-1
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed. F4/80 modulates cytokine levels released in response to Listeria monocytogenes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat - 1:10 Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from HOB2 rats. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice