397047
CSF1R (CD115, CSF-1R) (FITC)
Size
£754.00
- SKU:
- 397047
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant fusion protein from porcine CSF1R.
- Clone:
- ROS8G11
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R. CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages. The development of the monoclonal antibody specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10, use 10ul to label 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Ba/F3 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice