397024
CD52 (SWC1, Swine Workshop Cluster 1) (FITC)
Size
£754.00
- SKU:
- 397024
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Porcine thymocytes.
- Clone:
- 11/305/44
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- Porcine CD52 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1. Further studies have demonstrated that SWC1 is the porcine orthologue to human CD52, which is expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets. Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte- macrophage differentiation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat 1:10 Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice