CD27 (SWC2, Swine Workshop Cluster 2) (PE)

Size

£832.00

SKU:: 397011
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Porcine peripheral blood monocytes.
Clone:: B30C7
Format:: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml dH2O. Labeled with R-Phycoerythrin (PE).
Extra Details:: In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however studies undertaken using the CD27 have been unable to detect expression of CD27 on porcine B-cells. Studies in humans have demonstrated CD27 to be involved in apoptosis, research in porcine has shown that porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells. Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and research utilizing CD27 has shown that this monoclonal antibody may be used to differentiate between subsets of porcine NK cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice