CD16-2 (FcRIV)

Size

£620.00

SKU:: 396995
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Fusion protein corresponding to mouse CD16-2, rat CD4.
Clone:: AT137
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: CD16-2 is a low affinity activatory receptor that binds to IgG2a and IgG2b with intermediate affinity. FcRIV has no affinity for IgG1 or IgG3 and therefore has a unique subclass specificity when compared to other Fc receptors. Expression of CD16-2 is restricted to cells of the myeloid lineage. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat 1:10 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from 129/Ola mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.