HPRT (Hypoxanthine Phosphoribosyltransferase 1, HGPRT, HPRT1)

Size

£438.00

SKU:: 396879
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-218 of human HPRT, expressed in E. coli.
Clone:: AT1D9
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1- pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.