FCER2, Recombinant, Human, aa48-321, His-Tag (Low Affinity Immunoglobulin Epsilon Fc Receptor Isoform A, BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF)

Size

£487.00

SKU:: 396865
Molecular Weight:: 32
Purity:: ≥90% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: FCER2, also known as low affinity immunoglobulin epsilon Fc receptor isoform a, is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily. It is a low affinity receptor for B cell specific antigen and IgE. It plays an essential role in the growth and differentiation of B cells, and in the regulation of IgE production. This protein also exists in a soluble secretion form and functions as a powerful cleavage-promoting growth factor. Increased levels of soluble CD23 / FCER2 lead to the recruitment of unaffected B cells in the presentation of antigen peptides to allergen-specific B cells. Recombinant human FCER2, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa48-321 of human FCER2, fused to His-Tag at C-terminal, expressed in Baculovirus (insect cell). Swiss/Uniprot Accession: P06734 Molecular Weight: ~32kD (283aa) Biological Activity: The ED50 range is ≤15ug/ml as measured by its binding ability in a functional ELISA with human IgE. Amino Acid Sequence: <ADP>DTTQSLK QLEERAARNV SQVSKNLESH HGDQMAQKSQ STQISQELEE LRAEQQRLKS QDLELSWNLN GLQADLSSFK SQELNERNEA SDLLERLREE VTKLRMELQV SSGFVCNTCP EKWINFQRKC YYFGKGTKQW VHARYACDDM EGQLVSIHSP EEQDFLTKHA SHTGSWIGLR NLDLKGEFIW VDGSHVDYSN WAPGEPTSRS QGEDCVMMRG SGRWNDAFCD RKLGAWVCDR LATCTPPASE GSAESMGPDS RPDPDGRLPT PSAPLHS<HHH HHH> Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice