EPXH1, Recombinant, Human, aa21-455, His-Tag (Epoxide Hydrolase 1, EPHX1, EPHX, EPOX, HYL1, MEH)

Size

£607.00

SKU:: 396863
Molecular Weight:: 51.5
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl , pH 8.0, 1mM DTT, 0.1M sodium chloride, 50% glycerol.
Extra Details:: EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa21-455 of human EPHX1, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~51.5kD (442aa), ~50-70kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: MRDKEETLPL EDGWWGPGTR SAAREDDSIR PFKVETSDEE IHDLHQRIDK FRFTPPLEDS CFHYGFNSNY LKKVISYWRN EFDWKKQVEI LNRYPHFKTK IEGLDIHFIH VKPPQLPAGH TPKPLLMVHG WPGSFYEFYK IIPLLTDPKN HGLSDEHVFE VICPSIPGYG FSEASSKKGF NSVATARIFY KLMLRLGFQE FYIQGGDWGS LICTNMAQLV PSHVKGLHLN MALVLSNFST LTLLLGQRFG RFLGLTERDV ELLYPVKEKV FYSLMRESGY MHIQCTKPDT VGSALNDSPV GLAAYILEKF STWTNTEFRY LEDGGLERKF SLDDLLTNVM LYWTTGTIIS SQRFYKENLG QGWMTQKHER MKVYVPTGFS AFPFELLHTP EKWVRFKYPK LISYSYMVRG GHFAAFEEPE LLAQDIRKFL SVLERQHHHH HH Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice