Ddt, Recombinant, Mouse, aa1-118, His-Tag (D-Dopachrome Decarboxylase, C78655)

Size

£512.00

SKU:: 396857
Molecular Weight:: 15.5
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.
Extra Details:: Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihyroxyindole(DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-118 of mouse Ddt, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~15.5kD (141aa), Confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice