396829
AKT1/3 (RAC-alpha Serine/Threonine-protein Kinase)
Size
£438.00
- SKU:
- 396829
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C/-20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-480 of human AKT1, expressed in insect cell.
- Clone:
- AT6D5
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
- Extra Details:
- AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum- starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Flow Cytometry, ELISA, Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice