4-Hydroxynonenal (4-Hydroxynonenal, 4-Hydroxy Nonenal (4-HNE), 4-Hydroxy Nonenal, 4-HNE, 4-Hydroxy Hexenal, HNE, 4-Hydroxy-2-Nonenal)

Size

£639.00

SKU:: 384953
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic 4-Hydroxynonenal, conjugated to KLH.
Clone:: 12F7
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Extra Details:: 4-Hydroxynonenal (4-HNE) is a highly reactive α,β-unsaturated aldehyde generated during lipid peroxidation of ω-6 polyunsaturated fatty acids. As a key electrophilic byproduct of oxidative stress, 4-HNE plays a dual role in cellular physiology and pathology, particularly within the central nervous system. At low concentrations, 4-HNE can activate adaptive stress responses, including the upregulation of antioxidant defense pathways. However, elevated levels of 4-HNE are cytotoxic, promoting autophagy, apoptosis, and ultimately necrosis. Its reactivity with nucleophilic amino acid residues—especially cysteine, histidine, and lysine—leads to the formation of stable protein adducts that impair enzymatic activity, disrupt signaling pathways, and compromise cellular integrity. In neuroscience, 4-HNE is increasingly recognized as a critical factor in the pathogenesis of neurodegenerative diseases. Accumulation of 4-HNE-modified proteins has been observed in Alzheimer’s disease, Parkinson’s disease, and other age-related neurodegenerative disorders. These modifications contribute to mitochondrial dysfunction, synaptic failure, and chronic neuroinflammation—hallmarks of progressive neuronal decline. Beyond the brain, 4-HNE is implicated in a wide range of oxidative stress-related conditions, including cancer, cardiovascular disease, diabetes, and liver disorders. Its role as both a biomarker and a mediator of oxidative damage makes 4-HNE a compelling target for therapeutic intervention and early disease detection. Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 for detection of 4-Hydroxynonenal in 0.5ug of 4-Hydroxynonenal (BSA) by ECL immunoblot analysis using Goat Anti-Mouse IgG (HRP) as the secondary Antibody. Immunocytochemistry/Immunofluorescence: 1:50 ELISA: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice