FIG4/SAC3 (Polyphosphoinositide Phosphatase, 5-Bisphosphate 5-Phosphatase, Fig4, KIAA0274, Sac3, Phosphatidylinositol 3, Phosphatidylinositol 3,5 Bisphosphate 5 Phosphatase, SAC Domain Containing Protein 3, SAC Domain-Containing Protein 3, SAC3)

Size

£626.00

SKU:: 384920
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein corresponding to aa688-907 of mouse Sac3, at C-terminal. Species sequence homology: human (aa201/220) (91%), rat (aa203/220) (95%)
Clone:: S202-7
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Extra Details:: Fig4/Sac3 phosphatase, a 97kD protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles. It is broadly expressed in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signaling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy. Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice