NrCAM (Neuronal Cell Adhesion Molecule, Bravo, hBravo, KIAA0343, Neuronal Surface Protein Bravo, Ng CAM Related, Ng-CAM-Related, NgCAM Related Cell Adhesion Molecule, NgCAM-Related Cell Adhesion Molecule, Nr CAM, Nr-CAM)

Size

£626.00

SKU:: 384914
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein corresponding to aa30-845 of extracellular domain mouse NrCAM. Species sequence homology: rat (aa795/822) (96%), human (aa753/822) (91%).
Clone:: S364-51
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Extra Details:: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry (IC-IF): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice