CD74 (DHLAG, HLA DR Gamma, HLADG, p35, Protein 41, CD74, CD74 Antigen (Invariant Polypeptide Of Major Histocompatibility Complex, Class II Antigen-Associated), CD74 Antigen, CD74 Molecule, CD74 Molecule, Major Histocompatibility Complex, Class II Invariant Chain, CLIP, DHLAG, Gamma Chain of Class II Antigens, HG2A HUMAN, HLA Class II Histocompatibility Antigen Gamma Chain, HLA DR Antigens Associated Invariant Chain, HLA DR Gamma, HLA-DR Antigens-Associated Invariant Chain, HLA-DR-Gamma, HLADG, HLADR Antigens Associated Invariant Chain Ia Antigen Associated Invariant Chain, Ia Antigen-Associated Invariant Chain, Ia Associated Invariant Chain, Ia Gamma, Ii, Invariant Polypeptide Of Major Histocompatibility Complex Class II Antigen Associated, La-Gamma, Major Histocompatibility Complex Class II Invariant Chain, MHC HLA DR Gamma Chain, MHC HLA-DR Gamma Chain, p35, Protein 41)

Size

£626.00

SKU:: 384892
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to invariant chain human CD74.
Clone:: 1B8
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Extra Details:: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28aa, followed by a single 24aa transmembrane region and an approximately 150aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4). Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice