CENP-A (CENP A, Cenpa, Centromere Auto Antigen A, Histone H3 Like Centromeric Protein A)

Size

£576.00

SKU:: 384873
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to a portion of human CENP-A.
Clone:: 5A7-2E11
Format:: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Extra Details:: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome. Applications: Suitable for use in Western Blot and Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunofluorescence (IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice