381726
UHRF1, NT (Ac2-121, E3 Ubiquitin-Protein Ligase UHRF1, Hnp95 Huhrf1, HuNp95, ICBP90, Inverted CCAAT Box Binding Protein Of 90kD, Inverted CCAAT Box Binding Protein, 90kD, Inverted CCAAT Box-Binding Protein Of 90kD, Liver Regeneration-Related Protein LRRG126, NP95, Nuclear Phosphoprotein, 95-KD, Nuclear Protein 95, Nuclear Zinc Finger Protein Np95, RING Finger Protein 106, RNF106, Transcription Factor ICBP90, Ubiquitin Like Containing PHD And RING Finger Domains Protein 1, Ubiquitin Like PHD And RING Finger Domain Containing Protein 1, Ubiquitin-Like PHD And RING Finger Domain-Containing Protein 1, Ubiquitin-Like Protein Containing PHD And RING Finger Domains 1, Ubiquitin-Like With PHD And Ring Finger Domains 1, Ubiquitin-Like, Containing PHD And RING Finger Domains, 1, Ubiquitin-Like-Containing PHD And RING Finger Domains Protein 1)
Size
£633.00
- SKU:
- 381726
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to UHRF1.
- Clone:
- 17J148
- Format:
- Supplied as a liquid in 0.1M Tris-HCl, pH 7.4, 150mM sodium chloride, 50% glycerol.
- Extra Details:
- Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice