HP1-Gamma (CBX 3, CBX3, CBX3 HUMAN, Chromobox Homolog 3 (HP1 Gamma Homolog, Drosophila), Chromobox Homolog 3, Chromobox Protein Homolog 3, GAMMA, HECH, Heterochromatin Like Protein 1, Heterochromatin Protein 1 Homolog Gamma, Heterochromatin Protein HP1 Gamma, HP1 Gamma, HP1 Gamma Homolog, HP1Hs Gamma, Modifier 2 Protein)

Size

£633.00

SKU:: 381628
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to HP1-Gamma.
Clone:: 17J67
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the mehtyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Applications: Suitable for use in Western Blot, Immnoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:500 Immunocytochemistry: 1:200 Immunohistochemistry (FFPE):1:100 Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice