HP1 Alpha (Antigen p25, Cbx 5, Cbx5, CBX5 HUMAN, CG8409, Chromobox Homolog 5 (Drosophila HP1 Alpha), Chromobox Homolog 5 (HP1 Alpha Homolog Drosophila), Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila), Chromobox Homolog 5, Chromobox Protein Homolog 5, Chromobox Protein Homologue 5, Heterochromatin Protein 1 Alpha, Heterochromatin Protein 1, Heterochromatin Protein 1 Homolog Alpha, HP 1, HP 1A, HP1 Alpha, HP1 Alpha Homolog, HP1, HP1A, HP1Hs Alpha, Su(Var)205)

Size

£633.00

SKU:: 381627
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to HP1 Alpha.
Clone:: 17J66
Format:: Supplied as a liquid in 0.1M Tris-HCl, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry: 1:300 Immunohistochemistry:1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.