E2F-1 (Transcription Factor E2F1, E2F-1, Retinoblastoma Binding Protein 3, RBBP-3, PRB-Binding Protein E2F-1, PBR3, Retinoblastoma-Associated Protein 1, RBAP-1, RBBP3)

Size

£633.00

SKU:: 381606
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human E2F-1.
Clone:: 17J45
Format:: Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Extra Details:: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Immunocytochemistry: 1:100 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.