DBC1 (DBCCR1, Deleted In Bladder Cancer Protein 1, Deleted In Bladder Cancer Protein 1 Recursor, FAM5A, Protein FAM5A)

Size

£633.00

SKU:: 381602
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to DBC1.
Clone:: 17J41
Format:: Supplied as a liquid in 0.1M Tris-HCl, pH 7.4, 150mM sodium chloride, 0.02% sodium chloride, 50% glycerol.
Extra Details:: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3- specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation. Applications: Suitable for use in Western Blot, Immnoprecipitation, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Immunoprecipitation: 1:500 Immunocytochemistry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice