Globo H Ceramide (GHCer)

Size

£824.00

SKU:: 378338
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Globo H-KLH conjugate
Clone:: VK9
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Glycosphingolipids (GSLs) are a heterogeneous class of membrane glycolipids consisting of a ceramide backbone covalently linked to a glycan moiety and are involved in many cellular processes. Globo H (Fuc 1 2Galβ1 3GalNAcβ13Gal 14Galβ1 4Glc) is a carbohydrate structure, which is synthesized by specific glycosyltransferases, and shows enhanced expression in many human carcinomas. Globo H-ceramide (GHCer) is a glycosphingolipid and consists of a ceramide backbone with a carbohydrate chain that includes the Globo H, and serves as a critical tumor-associated carbohydrate antigen. It is predominantly expressed in various epithelial cancers, including breast, pancreatic, and colorectal cancers. GHCer is involved in cell signaling pathways that regulate tumor progression, immune evasion, and angiogenesis. Its expression is notably upregulated in malignant cells compared to normal tissues, making it a potential biomarker for cancer diagnosis and prognosis. Subcellularly, Globo H-ceramide localizes in lipid rafts and membrane microdomains, facilitating interactions with signaling molecules and receptors. Its involvement in disease is highlighted by its role in promoting cancer cell proliferation and metastasis, as well as its potential as a target for immunotherapeutic strategies aimed at enhancing anti-tumor immunity. Applications: Suitable for use in ELISA, Dot Blot, Electron Microscopy, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Inhibition Assays. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:50 detected Globo H-ceramide (GHCer) in human liver cancer tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice