Uchl1, Recombinant, Mouse, aa1-223, His-Tag (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1)

Size

£371.00

SKU:: 377997
Molecular Weight:: 27.2
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, 1mM DTT, 10% glycerol.
Extra Details:: Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Source: Recombinant protein corresponding to aa24-188 from mouse Uchl1, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~27.2kD (246aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >70pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1pmole of ubiquitin-AMC/minute at pH 7.5, at 37°C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQLKPME INPEMLNKVL AKLGVAGQWR FADVLGLEEE TLGSVPSPAC ALLLLFPLTA QHENFRKKQI EELKGQEVSP KVYFMKQTIG NSCGTIGLIH AVANNQDKLE FEDGSVLKQF LSETEKLSPE DRAKCFEKNE AIQAAHDSVA QEGQCRVDDK VNFHFILFNN VDGHLYELDG RMPFPVNHGA SSEDSLLQDA AKVCREFTER EQGEVRFSAV ALCKAA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice