TRAIL, Recombinant, Human, aa114-281 (TNF-Related Apoptosis Inducing Ligand, TNFSF10, Apo-2L, APO2L, CD253, TL2)

Size

£466.00

SKU:: 377993
Molecular Weight:: 19.6
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM DTT, 300mM sodium chloride, 10% glycerol.
Extra Details:: TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumor cell lines upon binding to the death-signalling receptors on the cell membrane. TRAIL binds to one of four receptors that have been identified in humans, including TRAIL-R1/DR4, TRAIL- R2/KILLER/DR5, TRAIL-R3/DcR1/TRID, and TRAIL-R4/DcR2/TRUNDD. Both DR4 and DR5 are pro-apoptotic receptors, which contain a cytoplasmic death domain and mediate apoptosis on binding to TRAIL. By contrast, TRID and TRUNDD do not contain a cytoplasmic death domain and block the function of TRAIL by competing with DR4 and DR5 for binding of TRAIL. Recombinant protein corresponding to aa114-281 from human TRAIL, expressed in E. coli. Molecular Weight: ~19.6kD (169aa) Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Measured in a cell cytotoxicity assay using Jurkat human T lymphocyte. The ED50 for this effect is ≤1ng/ml. AA Sequence: MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNG ELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS KDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice