PHPT1, Recombinant, Human, aa1-125, His-Tag (14kD Phosphohistidine Phosphatase, Phosphohistidine Phosphatase 1, Protein Janus-A Homolog, PHPT1, PHP14, CGI-202, HSPC141)

Size

£371.00

SKU:: 377924
Molecular Weight:: 15.9
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.2M sodium chloride, 10% glycerol.
Extra Details:: PHPT1 is a 125aa enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Source: Recombinant protein corresponding to aa1-125 from human PHPT1, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~15.9kD (145aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >120units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate/minute at pH 7.5 at 37°C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice