PDIA3, Recombinant, Mouse, aa25-505, His-Tag (Protein Disulfide-isomerase A3, 58kD, Erp, ERp57, ERp60, ERp61, Grp58, PDI, PDI-Q2, PI-PLC, Plca, PLC[a])

Size

£351.00

SKU:: 377916
Molecular Weight:: 56.8
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Extra Details:: PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Source: Recombinant protein corresponding to aa24-188 from mouse PDIA3, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~56.8kD (505aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >15 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNIE SLVKEYDDNG EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE QFLQEYFDGN LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK AQEDL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice