Leptin (Ob, Obese Protein, Obesity Factor)

Size

£438.00

SKU:: 377884
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa22-167 from human Leptin expressed in E. coli.
Clone:: AT7E10
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: Human leptin is a 16kD nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, ELISA, Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.