EPOR, Recombinant, Human, aa25-250, His-Tag (EPO-R, Erythropoietin Receptor)

Size

£371.00

SKU:: 377801
Molecular Weight:: 25.6
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase. Source: Recombinant protein corresponding to aa25-250 from human EPOR, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~25.6kD (232aa), 28-40kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Measured by its ability to inhibit EPO dependent proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ≤70ng/ml. Amino Acid Sequence: APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice