DCXR, Recombinant, Human, aa1-244, His-Tag (Dicarbonyl/L-xylulose Reductase, DCR, HCR2, HCRII, KIDCR, P34H)

Size

£371.00

SKU:: 377783
Molecular Weight:: 28
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 50mM sodium chloride, 20% glycerol.
Extra Details:: Dicarbonyl/L-xylulose reductase, also known as DCXR, is an enzyme responsible for the metabolism of xylulose, converting it into xylitol. DCXR was expressed at low levels and was localized predominantly in the cytoplasmic membrane. In contrast, in virtually all grades of early-stage prostate cancer and in all chemohormonally treated cases, DCXR was strikingly overexpressed and was localized predominantly in the cytoplasm and nucleus. Source: Recombinant protein corresponding to aa1-244 from human DCXR, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~28kD (264aa), confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >1800pmol/min/ug and is defined as the amount of enzyme that oxidize 1pmole of xylitol to L-xylulose/minute at pH 10.0 at 37°C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MELFLAGRRV LVTGAGKGIG RGTVQALHAT GARVVAVSRT QADLDSLVRE CPGIEPVCVD LGDWEATERA LGSVGPVDLL VNNAAVALLQ PFLEVTKEAF DRSFEVNLRA VIQVSQIVAR GLIARGVPGA IVNVSSQCSQ RAVTNHSVYC STKGALDMLT KVMALELGPH KIRVNAVNPT VVMTSMGQAT WSDPHKAKTM LNRIPLGKFA EVEHVVNAIL FLLSDRSGMT TGSTLPVEGG FWAC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice