BPGM (Bisphosphoglycerate Mutase)

Size

£438.00

SKU:: 377718
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-259 from human BPGM expressed in E. coli.
Clone:: AT2E11
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Extra Details:: Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2,3-BPG concentration. It is responsible for the catalytic synthesis of 2,3-Bisphosphoglycerate (2,3-BPG) from 1,3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry/Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1:100 Flow Cytometry: 2-5ug/ml to label 1x10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.