NF-kappaB1 p105/p50

Size

£626.00

SKU:: 376971
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Ile415 of mouse NF-kappaB1 p105/ p50.
Clone:: D4P4D
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: Transcription factors of the nuclear factor kappaB (NF-kappaB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kappaB1 (p105/p50), and NF-kappaB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kappaB is sequestered in the cytoplasm by IkappaB inhibitory proteins (3-5). NF-kappaB-activating agents can induce the phosphorylation of IkappaB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kappaB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kappaB2 (p100) to produce p52, which translocates to the nucleus (9-11). Following IKK-mediated phosphorylation of p105 NF-kappaB at multiple sites (Ser921, 923, 927, and 932) on its C-terminal, SCF/beta-TrCP-mediated processing produces the 50kD active form p50 (12,13). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, ChIP, Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Immunofluorescence (IF-IC): 1:200 ChIP: 1:50 Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice