376939
Caveolin-1 (CAV1)
Size
£581.00
- SKU:
- 376939
- Purity:
- Purified by Protein A and Peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Glu20 of human Caveolin-1.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The 21-24kD integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolins family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as G-a subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:400 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:250 Immunohistochemistry (frozen): 1:1000 Flow Cytometry: 1:50 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice